Review



089y cd45 30 f11 fluidigm 3089005b 142nd cxcr5 cd185 614641 fluidigm 3142008b 145nd cd69 h1 2 f3 fluidigm 3145005b 146nd cd8a  (fluidigm)


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    Structured Review

    fluidigm 089y cd45 30 f11 fluidigm 3089005b 142nd cxcr5 cd185 614641 fluidigm 3142008b 145nd cd69 h1 2 f3 fluidigm 3145005b 146nd cd8a
    089y Cd45 30 F11 Fluidigm 3089005b 142nd Cxcr5 Cd185 614641 Fluidigm 3142008b 145nd Cd69 H1 2 F3 Fluidigm 3145005b 146nd Cd8a, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluidigm+cd45+089y/Anti-Mouse+CD45+(30-F11)-89Y/pmc11461939__41467_2024_52714_MOESM1_ESM-53-6-9
    Average 93 stars, based on 34 article reviews
    089y cd45 30 f11 fluidigm 3089005b 142nd cxcr5 cd185 614641 fluidigm 3142008b 145nd cd69 h1 2 f3 fluidigm 3145005b 146nd cd8a - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Natural killer cell function is regulated by TGF-β signaling in pregnancy and tumor progression
    Article Snippet: Frozen samples from pregnant donors and healthy controls were thawed and stained for Cytof.thawed and stained for Cytof. ... The Cytof panel included the listed antibodies purchased from Fluidigm: CD45-089Y (3089003B), CD196-141Pr (3141003A), CD19-142Nd (3142001B), CD117-143Nd (3143001B), CD4-145Nd (3145001B),CD8a-146Nd (3146001B), CD25-149Sm (3149010B), CD103-151Eu (3151011B), TCRgd-152Sm (3152008B), TIGIT-153Eu (3153019B), CD3-154Sm (3154003B), CD194-158Gd (3158032A), CD161-164Dy (3164009B), CD39-160Gd (3160004B), CD27-162Dy (3162009B), CD45RO-165Ho (3165011B), CD127-168Er (3168017B), CD159a-169Tm (3169013B), CD45RA-170Er (3170010B), CD226-171Yb (3171013B); CD94-174Yb (3174015B), CD14-175Lu (3175015B), CD56-176Yb (3176009B); CD16 −299Bi (3209002B), CD7-147Sm (3147006B), PD1-155Gd (3155009B), CD26-161Dy (3161015B), CD57-163Dy (3163022B), CD158b-173Yb (3173010B), ICOS-148Nd (3148019B), CXCR3-156Gd (3156004B), CCR7-159Tb (3159003A), FceRI and CD138-150Nd (3150027B; 3150012B).. NKp44-167Er, which was conjugated in-house (eBioscience, 16-3369-85), was also included in the panel.NKp44-167Er, which was conjugated in-house..

    Article Title: Single-cell analyses of Crohn’s disease tissues reveal intestinal intraepithelial T cells heterogeneity and altered subset distributions
    Article Snippet: CyTOF: NKp44-167Er (eBioscience, 16-3369-85).CyTOF: NKp44-167Er (eBioscience, 16-3369-85).. Fluidigm: CD45-089Y (3089003B); CD196-141Pr (3141003A); CD19-142Nd (3142001B); CD117-143Nd (3143001B); CD4-145Nd (3145001B); CD8a-146Nd (3146001B); CD25-149Sm (3149010B); FceR-150Nd (3150027B); CD138-150Nd (3150012B); CD103-151Eu (3151011B); TCRgd-152Sm (3152008B); TIGIT-153Eu (3153019B); CD3-154Sm (3154003B); CD85j-156Gd (3156020B); CD194-158Gd (3158032A); CD161-159Tb (3159004B); CD39-160Gd (3160004B); CD27-162Dy (3162009B); CD45RO-165Ho (3165011B); CD34-166Er (3166012B); CD127-168Er (3168017B); CD159-169Tm (3169013B); CD45RA-170Er(3170010B); CD226-171Yb (3171013B); CD354(TREM1)-172Yb (3172022B); CD94-174Yb (3174015B); CD14-175Lu (3175015B); CD56-176Yb (3176009B) and CD16-209Bi (3209002B).. CD303-147Sm (3147009B); CD1b-155Gd (3155007B); DR3-161Dy (3161003B); CD294-163Dy (3163003B); CD141-173Yb (3173002B).CD303-147Sm (3147009B); CD1b-155Gd (3155007B); DR3-161Dy (3161003B); CD294-163Dy (3163003B); CD141-173Yb (3173002B).

    Staining:

    Article Title: Natural killer cell function is regulated by TGF-β signaling in pregnancy and tumor progression
    Article Snippet: Frozen samples from pregnant donors and healthy controls were thawed and stained for Cytof.thawed and stained for Cytof. ... The Cytof panel included the listed antibodies purchased from Fluidigm: CD45-089Y (3089003B), CD196-141Pr (3141003A), CD19-142Nd (3142001B), CD117-143Nd (3143001B), CD4-145Nd (3145001B),CD8a-146Nd (3146001B), CD25-149Sm (3149010B), CD103-151Eu (3151011B), TCRgd-152Sm (3152008B), TIGIT-153Eu (3153019B), CD3-154Sm (3154003B), CD194-158Gd (3158032A), CD161-164Dy (3164009B), CD39-160Gd (3160004B), CD27-162Dy (3162009B), CD45RO-165Ho (3165011B), CD127-168Er (3168017B), CD159a-169Tm (3169013B), CD45RA-170Er (3170010B), CD226-171Yb (3171013B); CD94-174Yb (3174015B), CD14-175Lu (3175015B), CD56-176Yb (3176009B); CD16 −299Bi (3209002B), CD7-147Sm (3147006B), PD1-155Gd (3155009B), CD26-161Dy (3161015B), CD57-163Dy (3163022B), CD158b-173Yb (3173010B), ICOS-148Nd (3148019B), CXCR3-156Gd (3156004B), CCR7-159Tb (3159003A), FceRI and CD138-150Nd (3150027B; 3150012B).. NKp44-167Er, which was conjugated in-house (eBioscience, 16-3369-85), was also included in the panel.NKp44-167Er, which was conjugated in-house..

    Article Title: Single-cell analyses of Crohn’s disease tissues reveal intestinal intraepithelial T cells heterogeneity and altered subset distributions
    Article Snippet: CyTOF: NKp44-167Er (eBioscience, 16-3369-85).CyTOF: NKp44-167Er (eBioscience, 16-3369-85).. Fluidigm: CD45-089Y (3089003B); CD196-141Pr (3141003A); CD19-142Nd (3142001B); CD117-143Nd (3143001B); CD4-145Nd (3145001B); CD8a-146Nd (3146001B); CD25-149Sm (3149010B); FceR-150Nd (3150027B); CD138-150Nd (3150012B); CD103-151Eu (3151011B); TCRgd-152Sm (3152008B); TIGIT-153Eu (3153019B); CD3-154Sm (3154003B); CD85j-156Gd (3156020B); CD194-158Gd (3158032A); CD161-159Tb (3159004B); CD39-160Gd (3160004B); CD27-162Dy (3162009B); CD45RO-165Ho (3165011B); CD34-166Er (3166012B); CD127-168Er (3168017B); CD159-169Tm (3169013B); CD45RA-170Er(3170010B); CD226-171Yb (3171013B); CD354(TREM1)-172Yb (3172022B); CD94-174Yb (3174015B); CD14-175Lu (3175015B); CD56-176Yb (3176009B) and CD16-209Bi (3209002B).. CD303-147Sm (3147009B); CD1b-155Gd (3155007B); DR3-161Dy (3161003B); CD294-163Dy (3163003B); CD141-173Yb (3173002B).CD303-147Sm (3147009B); CD1b-155Gd (3155007B); DR3-161Dy (3161003B); CD294-163Dy (3163003B); CD141-173Yb (3173002B).

    Derivative Assay:

    Article Title: Natural killer cell function is regulated by TGF-β signaling in pregnancy and tumor progression
    Article Snippet: Frozen samples from pregnant donors and healthy controls were thawed and stained for Cytof.thawed and stained for Cytof. ... The Cytof panel included the listed antibodies purchased from Fluidigm: CD45-089Y (3089003B), CD196-141Pr (3141003A), CD19-142Nd (3142001B), CD117-143Nd (3143001B), CD4-145Nd (3145001B),CD8a-146Nd (3146001B), CD25-149Sm (3149010B), CD103-151Eu (3151011B), TCRgd-152Sm (3152008B), TIGIT-153Eu (3153019B), CD3-154Sm (3154003B), CD194-158Gd (3158032A), CD161-164Dy (3164009B), CD39-160Gd (3160004B), CD27-162Dy (3162009B), CD45RO-165Ho (3165011B), CD127-168Er (3168017B), CD159a-169Tm (3169013B), CD45RA-170Er (3170010B), CD226-171Yb (3171013B); CD94-174Yb (3174015B), CD14-175Lu (3175015B), CD56-176Yb (3176009B); CD16 −299Bi (3209002B), CD7-147Sm (3147006B), PD1-155Gd (3155009B), CD26-161Dy (3161015B), CD57-163Dy (3163022B), CD158b-173Yb (3173010B), ICOS-148Nd (3148019B), CXCR3-156Gd (3156004B), CCR7-159Tb (3159003A), FceRI and CD138-150Nd (3150027B; 3150012B).. NKp44-167Er, which was conjugated in-house (eBioscience, 16-3369-85), was also included in the panel.NKp44-167Er, which was conjugated in-house..

    Article Title: Single-cell analyses of Crohn’s disease tissues reveal intestinal intraepithelial T cells heterogeneity and altered subset distributions
    Article Snippet: CyTOF: NKp44-167Er (eBioscience, 16-3369-85).CyTOF: NKp44-167Er (eBioscience, 16-3369-85).. Fluidigm: CD45-089Y (3089003B); CD196-141Pr (3141003A); CD19-142Nd (3142001B); CD117-143Nd (3143001B); CD4-145Nd (3145001B); CD8a-146Nd (3146001B); CD25-149Sm (3149010B); FceR-150Nd (3150027B); CD138-150Nd (3150012B); CD103-151Eu (3151011B); TCRgd-152Sm (3152008B); TIGIT-153Eu (3153019B); CD3-154Sm (3154003B); CD85j-156Gd (3156020B); CD194-158Gd (3158032A); CD161-159Tb (3159004B); CD39-160Gd (3160004B); CD27-162Dy (3162009B); CD45RO-165Ho (3165011B); CD34-166Er (3166012B); CD127-168Er (3168017B); CD159-169Tm (3169013B); CD45RA-170Er(3170010B); CD226-171Yb (3171013B); CD354(TREM1)-172Yb (3172022B); CD94-174Yb (3174015B); CD14-175Lu (3175015B); CD56-176Yb (3176009B) and CD16-209Bi (3209002B).. CD303-147Sm (3147009B); CD1b-155Gd (3155007B); DR3-161Dy (3161003B); CD294-163Dy (3163003B); CD141-173Yb (3173002B).CD303-147Sm (3147009B); CD1b-155Gd (3155007B); DR3-161Dy (3161003B); CD294-163Dy (3163003B); CD141-173Yb (3173002B).

    Gene Expression:

    Article Title: Natural killer cell function is regulated by TGF-β signaling in pregnancy and tumor progression
    Article Snippet: Frozen samples from pregnant donors and healthy controls were thawed and stained for Cytof.thawed and stained for Cytof. ... The Cytof panel included the listed antibodies purchased from Fluidigm: CD45-089Y (3089003B), CD196-141Pr (3141003A), CD19-142Nd (3142001B), CD117-143Nd (3143001B), CD4-145Nd (3145001B),CD8a-146Nd (3146001B), CD25-149Sm (3149010B), CD103-151Eu (3151011B), TCRgd-152Sm (3152008B), TIGIT-153Eu (3153019B), CD3-154Sm (3154003B), CD194-158Gd (3158032A), CD161-164Dy (3164009B), CD39-160Gd (3160004B), CD27-162Dy (3162009B), CD45RO-165Ho (3165011B), CD127-168Er (3168017B), CD159a-169Tm (3169013B), CD45RA-170Er (3170010B), CD226-171Yb (3171013B); CD94-174Yb (3174015B), CD14-175Lu (3175015B), CD56-176Yb (3176009B); CD16 −299Bi (3209002B), CD7-147Sm (3147006B), PD1-155Gd (3155009B), CD26-161Dy (3161015B), CD57-163Dy (3163022B), CD158b-173Yb (3173010B), ICOS-148Nd (3148019B), CXCR3-156Gd (3156004B), CCR7-159Tb (3159003A), FceRI and CD138-150Nd (3150027B; 3150012B).. NKp44-167Er, which was conjugated in-house (eBioscience, 16-3369-85), was also included in the panel.NKp44-167Er, which was conjugated in-house..

    Article Title: Single-cell analyses of Crohn’s disease tissues reveal intestinal intraepithelial T cells heterogeneity and altered subset distributions
    Article Snippet: CyTOF: NKp44-167Er (eBioscience, 16-3369-85).CyTOF: NKp44-167Er (eBioscience, 16-3369-85).. Fluidigm: CD45-089Y (3089003B); CD196-141Pr (3141003A); CD19-142Nd (3142001B); CD117-143Nd (3143001B); CD4-145Nd (3145001B); CD8a-146Nd (3146001B); CD25-149Sm (3149010B); FceR-150Nd (3150027B); CD138-150Nd (3150012B); CD103-151Eu (3151011B); TCRgd-152Sm (3152008B); TIGIT-153Eu (3153019B); CD3-154Sm (3154003B); CD85j-156Gd (3156020B); CD194-158Gd (3158032A); CD161-159Tb (3159004B); CD39-160Gd (3160004B); CD27-162Dy (3162009B); CD45RO-165Ho (3165011B); CD34-166Er (3166012B); CD127-168Er (3168017B); CD159-169Tm (3169013B); CD45RA-170Er(3170010B); CD226-171Yb (3171013B); CD354(TREM1)-172Yb (3172022B); CD94-174Yb (3174015B); CD14-175Lu (3175015B); CD56-176Yb (3176009B) and CD16-209Bi (3209002B).. CD303-147Sm (3147009B); CD1b-155Gd (3155007B); DR3-161Dy (3161003B); CD294-163Dy (3163003B); CD141-173Yb (3173002B).CD303-147Sm (3147009B); CD1b-155Gd (3155007B); DR3-161Dy (3161003B); CD294-163Dy (3163003B); CD141-173Yb (3173002B).

    Expressing:

    Article Title: Natural killer cell function is regulated by TGF-β signaling in pregnancy and tumor progression
    Article Snippet: Frozen samples from pregnant donors and healthy controls were thawed and stained for Cytof.thawed and stained for Cytof. ... The Cytof panel included the listed antibodies purchased from Fluidigm: CD45-089Y (3089003B), CD196-141Pr (3141003A), CD19-142Nd (3142001B), CD117-143Nd (3143001B), CD4-145Nd (3145001B),CD8a-146Nd (3146001B), CD25-149Sm (3149010B), CD103-151Eu (3151011B), TCRgd-152Sm (3152008B), TIGIT-153Eu (3153019B), CD3-154Sm (3154003B), CD194-158Gd (3158032A), CD161-164Dy (3164009B), CD39-160Gd (3160004B), CD27-162Dy (3162009B), CD45RO-165Ho (3165011B), CD127-168Er (3168017B), CD159a-169Tm (3169013B), CD45RA-170Er (3170010B), CD226-171Yb (3171013B); CD94-174Yb (3174015B), CD14-175Lu (3175015B), CD56-176Yb (3176009B); CD16 −299Bi (3209002B), CD7-147Sm (3147006B), PD1-155Gd (3155009B), CD26-161Dy (3161015B), CD57-163Dy (3163022B), CD158b-173Yb (3173010B), ICOS-148Nd (3148019B), CXCR3-156Gd (3156004B), CCR7-159Tb (3159003A), FceRI and CD138-150Nd (3150027B; 3150012B).. NKp44-167Er, which was conjugated in-house (eBioscience, 16-3369-85), was also included in the panel.NKp44-167Er, which was conjugated in-house..

    Article Title: Single-cell analyses of Crohn’s disease tissues reveal intestinal intraepithelial T cells heterogeneity and altered subset distributions
    Article Snippet: CyTOF: NKp44-167Er (eBioscience, 16-3369-85).CyTOF: NKp44-167Er (eBioscience, 16-3369-85).. Fluidigm: CD45-089Y (3089003B); CD196-141Pr (3141003A); CD19-142Nd (3142001B); CD117-143Nd (3143001B); CD4-145Nd (3145001B); CD8a-146Nd (3146001B); CD25-149Sm (3149010B); FceR-150Nd (3150027B); CD138-150Nd (3150012B); CD103-151Eu (3151011B); TCRgd-152Sm (3152008B); TIGIT-153Eu (3153019B); CD3-154Sm (3154003B); CD85j-156Gd (3156020B); CD194-158Gd (3158032A); CD161-159Tb (3159004B); CD39-160Gd (3160004B); CD27-162Dy (3162009B); CD45RO-165Ho (3165011B); CD34-166Er (3166012B); CD127-168Er (3168017B); CD159-169Tm (3169013B); CD45RA-170Er(3170010B); CD226-171Yb (3171013B); CD354(TREM1)-172Yb (3172022B); CD94-174Yb (3174015B); CD14-175Lu (3175015B); CD56-176Yb (3176009B) and CD16-209Bi (3209002B).. CD303-147Sm (3147009B); CD1b-155Gd (3155007B); DR3-161Dy (3161003B); CD294-163Dy (3163003B); CD141-173Yb (3173002B).CD303-147Sm (3147009B); CD1b-155Gd (3155007B); DR3-161Dy (3161003B); CD294-163Dy (3163003B); CD141-173Yb (3173002B).

    Incubation:

    Article Title: Natural killer cell function is regulated by TGF-β signaling in pregnancy and tumor progression
    Article Snippet: Frozen samples from pregnant donors and healthy controls were thawed and stained for Cytof.thawed and stained for Cytof. ... The Cytof panel included the listed antibodies purchased from Fluidigm: CD45-089Y (3089003B), CD196-141Pr (3141003A), CD19-142Nd (3142001B), CD117-143Nd (3143001B), CD4-145Nd (3145001B),CD8a-146Nd (3146001B), CD25-149Sm (3149010B), CD103-151Eu (3151011B), TCRgd-152Sm (3152008B), TIGIT-153Eu (3153019B), CD3-154Sm (3154003B), CD194-158Gd (3158032A), CD161-164Dy (3164009B), CD39-160Gd (3160004B), CD27-162Dy (3162009B), CD45RO-165Ho (3165011B), CD127-168Er (3168017B), CD159a-169Tm (3169013B), CD45RA-170Er (3170010B), CD226-171Yb (3171013B); CD94-174Yb (3174015B), CD14-175Lu (3175015B), CD56-176Yb (3176009B); CD16 −299Bi (3209002B), CD7-147Sm (3147006B), PD1-155Gd (3155009B), CD26-161Dy (3161015B), CD57-163Dy (3163022B), CD158b-173Yb (3173010B), ICOS-148Nd (3148019B), CXCR3-156Gd (3156004B), CCR7-159Tb (3159003A), FceRI and CD138-150Nd (3150027B; 3150012B).. NKp44-167Er, which was conjugated in-house (eBioscience, 16-3369-85), was also included in the panel.NKp44-167Er, which was conjugated in-house..

    Article Title: Single-cell analyses of Crohn’s disease tissues reveal intestinal intraepithelial T cells heterogeneity and altered subset distributions
    Article Snippet: CyTOF: NKp44-167Er (eBioscience, 16-3369-85).CyTOF: NKp44-167Er (eBioscience, 16-3369-85).. Fluidigm: CD45-089Y (3089003B); CD196-141Pr (3141003A); CD19-142Nd (3142001B); CD117-143Nd (3143001B); CD4-145Nd (3145001B); CD8a-146Nd (3146001B); CD25-149Sm (3149010B); FceR-150Nd (3150027B); CD138-150Nd (3150012B); CD103-151Eu (3151011B); TCRgd-152Sm (3152008B); TIGIT-153Eu (3153019B); CD3-154Sm (3154003B); CD85j-156Gd (3156020B); CD194-158Gd (3158032A); CD161-159Tb (3159004B); CD39-160Gd (3160004B); CD27-162Dy (3162009B); CD45RO-165Ho (3165011B); CD34-166Er (3166012B); CD127-168Er (3168017B); CD159-169Tm (3169013B); CD45RA-170Er(3170010B); CD226-171Yb (3171013B); CD354(TREM1)-172Yb (3172022B); CD94-174Yb (3174015B); CD14-175Lu (3175015B); CD56-176Yb (3176009B) and CD16-209Bi (3209002B).. CD303-147Sm (3147009B); CD1b-155Gd (3155007B); DR3-161Dy (3161003B); CD294-163Dy (3163003B); CD141-173Yb (3173002B).CD303-147Sm (3147009B); CD1b-155Gd (3155007B); DR3-161Dy (3161003B); CD294-163Dy (3163003B); CD141-173Yb (3173002B).

    Control:

    Article Title: Natural killer cell function is regulated by TGF-β signaling in pregnancy and tumor progression
    Article Snippet: Frozen samples from pregnant donors and healthy controls were thawed and stained for Cytof.thawed and stained for Cytof. ... The Cytof panel included the listed antibodies purchased from Fluidigm: CD45-089Y (3089003B), CD196-141Pr (3141003A), CD19-142Nd (3142001B), CD117-143Nd (3143001B), CD4-145Nd (3145001B),CD8a-146Nd (3146001B), CD25-149Sm (3149010B), CD103-151Eu (3151011B), TCRgd-152Sm (3152008B), TIGIT-153Eu (3153019B), CD3-154Sm (3154003B), CD194-158Gd (3158032A), CD161-164Dy (3164009B), CD39-160Gd (3160004B), CD27-162Dy (3162009B), CD45RO-165Ho (3165011B), CD127-168Er (3168017B), CD159a-169Tm (3169013B), CD45RA-170Er (3170010B), CD226-171Yb (3171013B); CD94-174Yb (3174015B), CD14-175Lu (3175015B), CD56-176Yb (3176009B); CD16 −299Bi (3209002B), CD7-147Sm (3147006B), PD1-155Gd (3155009B), CD26-161Dy (3161015B), CD57-163Dy (3163022B), CD158b-173Yb (3173010B), ICOS-148Nd (3148019B), CXCR3-156Gd (3156004B), CCR7-159Tb (3159003A), FceRI and CD138-150Nd (3150027B; 3150012B).. NKp44-167Er, which was conjugated in-house (eBioscience, 16-3369-85), was also included in the panel.NKp44-167Er, which was conjugated in-house..

    Article Title: Single-cell analyses of Crohn’s disease tissues reveal intestinal intraepithelial T cells heterogeneity and altered subset distributions
    Article Snippet: CyTOF: NKp44-167Er (eBioscience, 16-3369-85).CyTOF: NKp44-167Er (eBioscience, 16-3369-85).. Fluidigm: CD45-089Y (3089003B); CD196-141Pr (3141003A); CD19-142Nd (3142001B); CD117-143Nd (3143001B); CD4-145Nd (3145001B); CD8a-146Nd (3146001B); CD25-149Sm (3149010B); FceR-150Nd (3150027B); CD138-150Nd (3150012B); CD103-151Eu (3151011B); TCRgd-152Sm (3152008B); TIGIT-153Eu (3153019B); CD3-154Sm (3154003B); CD85j-156Gd (3156020B); CD194-158Gd (3158032A); CD161-159Tb (3159004B); CD39-160Gd (3160004B); CD27-162Dy (3162009B); CD45RO-165Ho (3165011B); CD34-166Er (3166012B); CD127-168Er (3168017B); CD159-169Tm (3169013B); CD45RA-170Er(3170010B); CD226-171Yb (3171013B); CD354(TREM1)-172Yb (3172022B); CD94-174Yb (3174015B); CD14-175Lu (3175015B); CD56-176Yb (3176009B) and CD16-209Bi (3209002B).. CD303-147Sm (3147009B); CD1b-155Gd (3155007B); DR3-161Dy (3161003B); CD294-163Dy (3163003B); CD141-173Yb (3173002B).CD303-147Sm (3147009B); CD1b-155Gd (3155007B); DR3-161Dy (3161003B); CD294-163Dy (3163003B); CD141-173Yb (3173002B).



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    a-d , Representative flow cytometry plots showing sorting strategy <t>(CD45</t> + , YFP - ) maternal immune cells after doublet exclusion: a , maternal immune cells from E14.5 maternal fetal interface; b , immune cells from E14.5 fetal liver; c , immune cells from E14.5 pregnant female Spleen; d , immune cells from E14.5 pregnant female peripheral blood. e , Total UMIs per single cell from each sorted 384-well plate. f , Fraction of analyzed cells after filtering per plate.
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    a-d , Representative flow cytometry plots showing sorting strategy <t>(CD45</t> + , YFP - ) maternal immune cells after doublet exclusion: a , maternal immune cells from E14.5 maternal fetal interface; b , immune cells from E14.5 fetal liver; c , immune cells from E14.5 pregnant female Spleen; d , immune cells from E14.5 pregnant female peripheral blood. e , Total UMIs per single cell from each sorted 384-well plate. f , Fraction of analyzed cells after filtering per plate.
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    a-d , Representative flow cytometry plots showing sorting strategy <t>(CD45</t> + , YFP - ) maternal immune cells after doublet exclusion: a , maternal immune cells from E14.5 maternal fetal interface; b , immune cells from E14.5 fetal liver; c , immune cells from E14.5 pregnant female Spleen; d , immune cells from E14.5 pregnant female peripheral blood. e , Total UMIs per single cell from each sorted 384-well plate. f , Fraction of analyzed cells after filtering per plate.
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    a-d , Representative flow cytometry plots showing sorting strategy <t>(CD45</t> + , YFP - ) maternal immune cells after doublet exclusion: a , maternal immune cells from E14.5 maternal fetal interface; b , immune cells from E14.5 fetal liver; c , immune cells from E14.5 pregnant female Spleen; d , immune cells from E14.5 pregnant female peripheral blood. e , Total UMIs per single cell from each sorted 384-well plate. f , Fraction of analyzed cells after filtering per plate.
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    a-d , Representative flow cytometry plots showing sorting strategy <t>(CD45</t> + , YFP - ) maternal immune cells after doublet exclusion: a , maternal immune cells from E14.5 maternal fetal interface; b , immune cells from E14.5 fetal liver; c , immune cells from E14.5 pregnant female Spleen; d , immune cells from E14.5 pregnant female peripheral blood. e , Total UMIs per single cell from each sorted 384-well plate. f , Fraction of analyzed cells after filtering per plate.
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    Image Search Results


    a-d , Representative flow cytometry plots showing sorting strategy (CD45 + , YFP - ) maternal immune cells after doublet exclusion: a , maternal immune cells from E14.5 maternal fetal interface; b , immune cells from E14.5 fetal liver; c , immune cells from E14.5 pregnant female Spleen; d , immune cells from E14.5 pregnant female peripheral blood. e , Total UMIs per single cell from each sorted 384-well plate. f , Fraction of analyzed cells after filtering per plate.

    Journal: bioRxiv

    Article Title: Natural killer cell function is regulated by TGF-β signaling in pregnancy and tumor progression

    doi: 10.1101/2024.09.18.613652

    Figure Lengend Snippet: a-d , Representative flow cytometry plots showing sorting strategy (CD45 + , YFP - ) maternal immune cells after doublet exclusion: a , maternal immune cells from E14.5 maternal fetal interface; b , immune cells from E14.5 fetal liver; c , immune cells from E14.5 pregnant female Spleen; d , immune cells from E14.5 pregnant female peripheral blood. e , Total UMIs per single cell from each sorted 384-well plate. f , Fraction of analyzed cells after filtering per plate.

    Article Snippet: The Cytof panel included the listed antibodies purchased from Fluidigm: CD45-089Y (3089003B), CD196-141Pr (3141003A), CD19-142Nd (3142001B), CD117-143Nd (3143001B), CD4-145Nd (3145001B),CD8a-146Nd (3146001B), CD25-149Sm (3149010B), CD103-151Eu (3151011B), TCRgd-152Sm (3152008B), TIGIT-153Eu (3153019B), CD3-154Sm (3154003B), CD194-158Gd (3158032A), CD161-164Dy (3164009B), CD39-160Gd (3160004B), CD27-162Dy (3162009B), CD45RO-165Ho (3165011B), CD127-168Er (3168017B), CD159a-169Tm (3169013B), CD45RA-170Er (3170010B), CD226-171Yb (3171013B); CD94-174Yb (3174015B), CD14-175Lu (3175015B), CD56-176Yb (3176009B); CD16 −299Bi (3209002B), CD7-147Sm (3147006B), PD1-155Gd (3155009B), CD26-161Dy (3161015B), CD57-163Dy (3163022B), CD158b-173Yb (3173010B), ICOS-148Nd (3148019B), CXCR3-156Gd (3156004B), CCR7-159Tb (3159003A), FceRI and CD138-150Nd (3150027B; 3150012B).

    Techniques: Flow Cytometry

    a-b , Representative flow cytometry plots showing sorting strategy (CD45 + ) for immune cells or NK (CD45 + , CD56 + , CD3 - ) after doublet exclusion. Samples are from human decidua tissue ( a ) and peripheral blood ( b ). Cells were stained for immune populations markers such as CD45, CD3 (T cells), and CD56 (NK cells). c , Total UMIs per single cell from each sorted 384-well plate. d , Fraction of analyzed cells after filtering per plate.

    Journal: bioRxiv

    Article Title: Natural killer cell function is regulated by TGF-β signaling in pregnancy and tumor progression

    doi: 10.1101/2024.09.18.613652

    Figure Lengend Snippet: a-b , Representative flow cytometry plots showing sorting strategy (CD45 + ) for immune cells or NK (CD45 + , CD56 + , CD3 - ) after doublet exclusion. Samples are from human decidua tissue ( a ) and peripheral blood ( b ). Cells were stained for immune populations markers such as CD45, CD3 (T cells), and CD56 (NK cells). c , Total UMIs per single cell from each sorted 384-well plate. d , Fraction of analyzed cells after filtering per plate.

    Article Snippet: The Cytof panel included the listed antibodies purchased from Fluidigm: CD45-089Y (3089003B), CD196-141Pr (3141003A), CD19-142Nd (3142001B), CD117-143Nd (3143001B), CD4-145Nd (3145001B),CD8a-146Nd (3146001B), CD25-149Sm (3149010B), CD103-151Eu (3151011B), TCRgd-152Sm (3152008B), TIGIT-153Eu (3153019B), CD3-154Sm (3154003B), CD194-158Gd (3158032A), CD161-164Dy (3164009B), CD39-160Gd (3160004B), CD27-162Dy (3162009B), CD45RO-165Ho (3165011B), CD127-168Er (3168017B), CD159a-169Tm (3169013B), CD45RA-170Er (3170010B), CD226-171Yb (3171013B); CD94-174Yb (3174015B), CD14-175Lu (3175015B), CD56-176Yb (3176009B); CD16 −299Bi (3209002B), CD7-147Sm (3147006B), PD1-155Gd (3155009B), CD26-161Dy (3161015B), CD57-163Dy (3163022B), CD158b-173Yb (3173010B), ICOS-148Nd (3148019B), CXCR3-156Gd (3156004B), CCR7-159Tb (3159003A), FceRI and CD138-150Nd (3150027B; 3150012B).

    Techniques: Flow Cytometry, Staining

    a , Heatmap of human NK cells derived from diverse tumors. Columns represent metacells, further aggregated into 6 clustered representing 3 distinct modules. Color-coded for log scaled gene expression. b , Same as a but for murine NK cells. c , Barplots showing expression fold change (log2 scale) of selected genes in cytotoxic and chemotactic TANKs compared with blood TANKs. d , Scaled gene expression of TGFb KO vs WT NK cells. e , Live fraction of K562 cells post incubation with NK cells, NK cells + TGFb, or idle. f , Fraction of NK cells from total CD45+ cells in anti-TGFb-treated and isotype control tumors. g , Tumor kinetics for mice treated with anti-TGFb and isotype control.

    Journal: bioRxiv

    Article Title: Natural killer cell function is regulated by TGF-β signaling in pregnancy and tumor progression

    doi: 10.1101/2024.09.18.613652

    Figure Lengend Snippet: a , Heatmap of human NK cells derived from diverse tumors. Columns represent metacells, further aggregated into 6 clustered representing 3 distinct modules. Color-coded for log scaled gene expression. b , Same as a but for murine NK cells. c , Barplots showing expression fold change (log2 scale) of selected genes in cytotoxic and chemotactic TANKs compared with blood TANKs. d , Scaled gene expression of TGFb KO vs WT NK cells. e , Live fraction of K562 cells post incubation with NK cells, NK cells + TGFb, or idle. f , Fraction of NK cells from total CD45+ cells in anti-TGFb-treated and isotype control tumors. g , Tumor kinetics for mice treated with anti-TGFb and isotype control.

    Article Snippet: The Cytof panel included the listed antibodies purchased from Fluidigm: CD45-089Y (3089003B), CD196-141Pr (3141003A), CD19-142Nd (3142001B), CD117-143Nd (3143001B), CD4-145Nd (3145001B),CD8a-146Nd (3146001B), CD25-149Sm (3149010B), CD103-151Eu (3151011B), TCRgd-152Sm (3152008B), TIGIT-153Eu (3153019B), CD3-154Sm (3154003B), CD194-158Gd (3158032A), CD161-164Dy (3164009B), CD39-160Gd (3160004B), CD27-162Dy (3162009B), CD45RO-165Ho (3165011B), CD127-168Er (3168017B), CD159a-169Tm (3169013B), CD45RA-170Er (3170010B), CD226-171Yb (3171013B); CD94-174Yb (3174015B), CD14-175Lu (3175015B), CD56-176Yb (3176009B); CD16 −299Bi (3209002B), CD7-147Sm (3147006B), PD1-155Gd (3155009B), CD26-161Dy (3161015B), CD57-163Dy (3163022B), CD158b-173Yb (3173010B), ICOS-148Nd (3148019B), CXCR3-156Gd (3156004B), CCR7-159Tb (3159003A), FceRI and CD138-150Nd (3150027B; 3150012B).

    Techniques: Derivative Assay, Gene Expression, Expressing, Incubation, Control